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Sterilization and disinfection of medical devices

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Sterilization and disinfection are two important processes in infection prevention and control. Sterilization is the process of killing all microorganisms, including bacterial spores, on an inanimate object. Disinfection is the process of reducing the number of microorganisms on an inanimate object to a safe level.

The level of sterilization or disinfection required depends on the intended use of the object. Medical devices that come into contact with sterile tissue or body fluids, such as surgical instruments and implants, must be sterile. Medical devices that come into contact with non-sterile tissue or body fluids, such as stethoscopes and blood pressure cuffs, must be disinfected.

Sterilization methods

There are a number of different sterilization methods, including:


  • Steam sterilization: Steam under pressure is the most common method of sterilization in healthcare settings. It is a reliable and effective way to kill all microorganisms, including bacterial spores.

  • Dry heat sterilization: Dry heat sterilization is used for items that cannot be exposed to steam, such as glassware and metal instruments. It is less effective than steam sterilization, but it is still effective against most microorganisms.

  • Chemical sterilization: Chemical sterilization is used for items that cannot be exposed to heat, such as some plastics and electronic devices. It is important to choose a chemical sterilant that is effective against the types of microorganisms that are likely to be present on the item.


Disinfection methods

There are a number of different disinfection methods, including:


  • Liquid chemical disinfection: Liquid chemical disinfectants are used to disinfect surfaces and equipment. They are available in a variety of formulations, including sprays, wipes, and immersion solutions.

  • Gaseous chemical disinfection: Gaseous chemical disinfectants, such as ethylene oxide and hydrogen peroxide gas plasma, are used to disinfect items that cannot be immersed in liquid disinfectants.

  • High-level disinfection: High-level disinfection is a process that kills all microorganisms except for bacterial spores. It is used for medical devices that come into contact with mucous membranes or non-intact skin.

  • Intermediate-level disinfection: Intermediate-level disinfection is a process that kills most microorganisms except for bacterial spores and some viruses. It is used for medical devices that come into contact with non-intact skin.

  • Low-level disinfection: Low-level disinfection is a process that kills some microorganisms, but not bacterial spores or some viruses. It is used for surfaces and equipment that are likely to come into contact with intact skin.


Infection prevention and control

Sterilization and disinfection are essential components of infection prevention and control programs in healthcare settings. By properly sterilizing and disinfecting medical devices and surfaces, healthcare workers can help to prevent the spread of infection to patients.

Here are some specific examples of how sterilization and disinfection are used to prevent infection:


  • Surgical instruments are sterilized before each use to prevent the spread of infection to the surgical site.

  • Endoscopes are high-level disinfected after each use to prevent the spread of infection to the next patient.

  • Patient care equipment, such as stethoscopes and blood pressure cuffs, is disinfected between patients to prevent the spread of infection.

  • Environmental surfaces, such as countertops and floors, are disinfected regularly to prevent the spread of infection to patients and staff.


Sterilization and disinfection are essential for preventing the spread of infection in healthcare settings. By following proper sterilization and disinfection procedures, healthcare workers can help to protect patients and staff from infection.

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